East Port + Promega seminar: Bioluminescent Technologies for Studying Protein Biology and Cellular Responses

Seminář
October 10

National library of Technology (Education center), Technická 2710/6, 160 80 Praha 6

October 11

Masaryk University in Brno, Department of Biochemistry (FSci), Kamenice 5, C05, 1. floor, lecture room 114

Brno 18.10.2023 (2.term)

Masaryk University in Brno, Department of Biochemistry (FSci), Kamenice 5, C05, 1. floor, lecture room 114

East Port + Promega seminar: Bioluminescent Technologies for Studying Protein Biology and Cellular Responses

This seminar introduces a comprehensive set of bioluminescent technologies based on the versatile NanoLuc® luciferase. Discover how the extraordinary brightness of this reporter benefits research in the field of cell biology. You will learn how to investigate protein interactions in live cells using the NanoBiT® technology and experience how the HiBiT Protein Tagging System simplifies studies on protein biology by enabling antibody-free detection and quantification of tagged proteins. The combination of HiBiT with CRISPR genome editing provides a tool to study proteins under their native expression conditions. Finally, experience the possibilities of Lumit™ Immunoassays, an innovative fast-track method for protein quantification and interaction analysis.
 
Main topics:
•Protein Interaction
•Viral Infection
•Target Cell Killing
•Cell Signaling
 

Programme of the event

The lecture schedule is the same for both dates. Only the date and place of the lecture differ.

10.10.2023 v 10:00
National library of Technology (Education center), Technická 2710/6, 160 80 Praha 6
Vzdělávací centrum NTK.jpgNTK_1.jpg
11.10.2023 v 10:00
Masaryk University in Brno, Department of Biochemistry (FSci), Kamenice 5, Department C05, 1. floor, lecture room 114
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10:00-10:20 Axion Biosystems – realtime monitoring of cell health and differentiation
10:20–11:00 NanoBiT technology for studying protein interactions
11:00-11:20 Coffee break
11:20-12:00 HiBiT and Lumit technologies for protein and cytokine quantification

Speakers

300x300px-erik-bonke.jpg Erik Bonke Field Application Specialist Promega

Erik Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.

Vojtěch ledvina aplikační specialista Vojtěch Ledvina aplikační specialista East Port

Vojtěch Ledvina received his master's degree in biotechnology at the Brno University of Technology and subsequently completed his doctoral studies in analytical chemistry at Masaryk University and the Institute of Analytical Chemistry of the CAS. He has been involved in, among other things, the study of cell differentiation by bioluminescence and the synthesis and functionalization of fluorescent nanoparticles. Since 2020, he has been working in Eastport as an application specialist over products from Promega.