Experimental RNA Workflow – From qPCR Basics to RNA interference
IMG, lecture room on the ground floor of the main building
IACH, Seminar room on the 5th floor, registration at the gatehouse
Experimental RNA Workflow – From qPCR Basics to RNA interference (Prague, Brno)
Presentations for download:
Prezentace RNA Workflow Kerem Yildirim Promega
Brochures for download:
Promega Genomic Essentials Products 2025/26
siPOOL-efficient-gene-silencing-with-siRNAs
Promega Maxwell RSC Instrument Brochure
Pro českou verzi, KLIKNĚTE ZDE.
We are pleased to invite you to our upcoming seminar, which will this time focus on RNA analysis across the entire experimental workflow.
The session will cover:
- Sample preparation techniques
- Real-time PCR (qPCR) basics and various setup options (probe-based vs dye-based)
- Data processing, analysis, and common troubleshooting strategies
In the second part of the seminar, we will explore:
- The fundamentals of RNA interference (RNAi)
- Targeted gene silencing using specific siRNA pools
- Methods of rRNA depletion for RNA sequencing (RNA-seq) experiments
Finally, we will introduce innovative and practical solutions for long-term RNA sample storage to help you preserve your valuable material.
Join us to gain insights, ask questions, and enhance your RNA-based research!
Schedule
| 10:00-11:00 | RNA analysis from isolation to qPCR troubleshooting (Kerem Yildirim) |
| 11:00-11:20 | Coffee Break |
| 11:20-11:50 | Studying gene function using complex siRNA pools (Vojtěch Ledvina) |
Who is the seminar for?
The seminar is open to all interested participants, regardless of their background or age, and is also suitable for university students. However, due to capacity and organizational reasons, registration is required for participation. The entire seminar will be conducted in English.
Speakers
Dr. Kerem Yildirim (Promega)
Dr. Kerem Yıldırım provides scientific expertise and supports the implementation of Promega’s innovative solutions and technologies, tailored to the specific goals of each customer’s research. His mission is to empower scientists with the right tools and resources to accelerate their discoveries and achieve impactful outcomes.
Dr. Vojtěch Ledvina (East Port)
Dr. Vojtěch Ledvina is an application specialist for our complete life science portfolio, namely cell assays, reporter assays, molecular biology, proteomics and scientific laboratory instruments. He is also responsible for organizing webinars and seminars introducing our suppliers' technologies, at which he lectures himself.
