East Port zve na seminář: NanoBiT Technology Platform

NanoBiT Technology Platform - Innovative Tools to Study Cellular Protein Biology

Come and learn more about exciting new tools and applications based on the NanoLuc® Technology such as NanoBiT and HiBiT. NanoLuc® is a versatile small and ultra-sensitive luciferase enabling you to perform reporter assays at physiologically relevant expression levels on challenging topics such as protein interactions. Join this seminar in order to familiarize with luciferase reporter assays in general and to learn about the advancements of the 2nd generation reporter technology based on NanoLuc®. Gain exciting insights into Promega’s upcoming HiBiT protein tag system - an ideal companion for CRISPR-mediated tagging of proteins at the endogenous level. The seminar will cover the following applications:

  • HiBiT protein tagging system +/- CRISPR
  • Autophagy LC3 HiBiT Reporter Assay
  • NanoBiT PPI
  • RealTime-Glo Annexin V Apoptosis and Necrosis Assay
  • Some general aspects of cell-based assays (detection modes, plate types etc.)
  • ImunnoBiT System for determination of pathway activation

The seminar is free of charge, but we require registration of each participant:

Registration, 26.3.2019 Praha, Institute of Organic Chemistry and Biochemistry (UOCHB)

Registration, 27.3.2019 Praha, Institute of Molecular Genetics (UMG)

The seminar will take place on Tuesday, at 10.00 a.m., March 26th 2019 in Prague in the Institute of Organic Chemistry and Biochemistry (conference hall, 2nd level, main building) and on Wednesday, at 10.00 a.m., March 27th 2019 in Prague in the Institute of Molecular Genetics (seminar room ground floor).  

Snacks and Beverage will be provided.




9:30 - 10:00

Check in

10:00 - 10:05


Part 1
Dr. Erik Bonke, Promega GmbH, Mannheim (Dr. Erik Bonke resume)

Coffee break


Part 2
Dr. Erik Bonke, Promega GmbH, Mannheim




Scientists, PhD students, clinical researchers, technical assistants and students are welcome !

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