East Port zve na seminář: Innovations in Bioluminescence

Innovative Applications of Cell-Based Luciferase Reporter Assays

Come and learn more about exciting new tools and applications based on the NanoLuc® Technology such as NanoBRET, NanoBiT and HiBiT. NanoLuc® is a versatile small and ultra-sensitive luciferase enabling you to perform reporter assays at physiologically relevant expression levels on challenging topics such as protein interactions. Join this seminar in order to familiarize with luciferase reporter assays in general and to learn about the advancements of the 2nd generation reporter technology based on NanoLuc®. Gain exciting insights into Promega’s upcoming HiBiT protein tag system – an ideal companion for CRISPR-mediated tagging of proteins at the endogenous level. The seminar will cover the following applications of luciferase reporters:

  • Cell Signaling
  • Protein:Protein Interaction
  • Biosensors
  • Real-Time Apoptosis
  • Protein Tagging and Quantification System

The seminar is free of charge, but we require registration of each participant:

Registration, 27.3.2018 Praha, IMG ASCR

Registration, 28.3.2018 Brno, Masaryk University


The seminar will take place on Tuesday, March 27th 2018 in Prague in the Institute of Molecular Genetics of the Academy of Sciences (seminar room on the ground floor behind lifts) and on Wednesday, March 28th 2018 in Brno at Masaryk University Campus (seminar room 114, first floor, building A5).  

Snacks and Beverage will be provided.

 

Program:

 

09:30 - 10:00

Check in

10:00 - 10:05

Welcome

10:05 - 10.50

NanoBiT® Technology  (HiBiT® Tagging System, Autophagy Assay, Protein:Protein Interaction Assays, Real-Time Apoptosis Assay)
Dr. Oleksii Ivanov, Promega GmbH, Mannheim

10:50 - 11:10

Coffee break

11:10 - 11:55

NanoBRETTM Technology (Protein:Protein Interactions, Protein:Ligand Interactions /  Target Engagement, BRET Sensor Proteins)
 
Dr. Oleksii Ivanov, Promega GmbH, Mannheim

12:00

End

 

Scientists, PhD students, clinical researchers, technical assistants and students are welcome !

 
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